Replication of Japanese Encephalitis Virus.

Abstract

Preliminary studies of the structure of Japanese encephalitis virion RNA indicated that the 5'-terminus had a 7-methyl-guanine cap and that the 3'-terminus had little or no polyadenylic acid. Definitive structural studies have not been completed due to difficulties in obtaining sufficient material. Two-dimensional electrophoretic maps of RNase T1-resistant oligonucleotides derived from virion RNA propagated in vertebrate and invertebrate cells were identical. Attempts were made to determine th 3'-terminal base sequence of virion RNA by end-labeling followed by base-specific chemical cleavage. The RNA accepted label poorly by comparison to RNAs from other sources, suggesting the presence of a unique 3'-terminal structure.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1981
Accession Number
ADA127174

Entities

People

  • Carol D. Blair

Organizations

  • Colorado State University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Antigens
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Coinfection
  • Culture Techniques
  • Cultured Cells
  • Department Of Defense
  • Encephalitis
  • Endoplasmic Reticulum
  • Mouth Diseases
  • Proteins
  • Standards
  • Vaccines
  • Viral Structures
  • Virion
  • Viruses

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Systems Analysis and Design
  • Virology (or Medical Virology).