Development and Use of Anucleate Bacterial Cells to Assay the in vitro Activity of Pollutants
Abstract
The T7 0.3 gene product (0.3 protein) was purified by a modification of the published procedure (2), and used to raise antibody to this protein. A radioimmune precipitation (RIP) assay was developed which could be used to estimate the increased misincorporation of cysteine into 0.3 protein. Parameters of the RIP assay were varied to make the RIP-polyacrylamide gel electrophoresis (RIP-PAGE) assay specific for the 0.3 protein. A single protein band was, however, never achieved although increased misincorporation of cysteine into the 0.3 protein can now be estimated by RIP-PAGE combined with scanning densitometry.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 07, 1982
- Accession Number
- ADA128378
Entities
People
- John N. Reeve
Organizations
- Ohio State University