Use of Dye-Ligand Affinity Chromatography for Separation of Toxin Components of Bacillus Anthracis

Abstract

The protective antigen and lethal factor components of anthrax toxin were isolated from Bacillus anthracis culture supernatants and separated using dye-ligand affinity chromatography. The two components were assayed for serological activity by immunodiffusion analysis, for molecular weight by sodium dodecyl sulfate polyacrylamide gel electrophoresis, and for biological activity by injection into Fisher 344 rats. They were shown to be serologically distinct, to possess different electrophoretic mobilities, and to demonstrate lethal toxic activity only when combined.

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Document Details

Document Type
Technical Report
Publication Date
May 18, 1983
Accession Number
ADA129470

Entities

People

  • Bruce E. Ivins
  • Dale W. Seburn
  • Joseph D. Ristroph
  • Kenneth W. Hedlund
  • Stephen F. Little

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acetic Acid
  • Animals
  • Biomedical Research
  • Chromatography
  • Diseases And Disorders
  • Electrophoresis
  • Gel Electrophoresis
  • High Pressure
  • Immune Serums
  • Infectious Diseases
  • Ion Exchange
  • Laboratory Animals
  • Liquid Chromatography
  • Materials
  • Molecular Weight
  • Public Health
  • Vaccines

Fields of Study

  • Biology

Readers

  • Immunology
  • Logistics and Supply Chain Management.
  • Molecular and Cellular Biochemistry