Pathogenesis of Dengue Vaccine Viruses in Mosquitoes.

Abstract

An in vitro assay was developed to detect transmission of Dengue-2 parent and vaccine virus strains by vector mosquitoes. After interthoracic inoculations, transmission of both viruses was detected at 7 and 14 days incubation. Rates of transmission were similar. A mosquito inoculation-headsquash-immunofluorescence technique was found to be more sensitive than Aedes albopictus cells (C6/36) for the isolation of dengue virus in the in vitro transmission assay. Flavivirus viremias in suckling mice proved relatively noninfectious for Aedes aegypti. LLC-MK2 cells were adapted to a medium containing calf serum in order to conserve fetal calf serum. Cells were maintained and grown in calf serum; fetal calf serum was used only in the overlay medium used in the plague assay test.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1980
Accession Number
ADA130518

Entities

People

  • Barry J. Beaty
  • Thomas H. G. Aitken

Organizations

  • Yale University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Antigens
  • Arboviruses
  • Biological Factors
  • Blood
  • Body Fluids
  • Cells
  • Detection
  • Infection
  • Laboratory Animals
  • Lagomorphs
  • Leukocytes
  • Pathogenesis
  • Rodents
  • Vaccines
  • Virus Diseases
  • Viruses
  • Yellow Fever

Fields of Study

  • Biology

Readers

  • Virology (or Medical Virology).

Technology Areas

  • Biotechnology