Pathogenesis of Dengue Vaccine Viruses in Mosquitoes.

Abstract

An artificial bloodmeal feeding technique was developed which resulted in infection rates of up to 100% in mosquitoes ingesting low passage or attenuated dengue-2 viruses. The technique was used in studies to compare the relative abilities of the PR-159 parent and S-1 vaccine dengue-2 viruses to infect and to be transmitted by Aedes albopictus and Aedes aegypti mosquitoes. The S-1 vaccine candidate virus was markedly less efficient than the PR-159 parent in infection of midgut cells, dissemination from midgut cells and infection of secondary target organs, and oral transmission by the vectors. In addition the vaccine strain replicated more slowly in the vectors than the parent strain.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1981
Accession Number
ADA130520

Entities

People

  • Barry J. Beaty
  • Thomas H. G. Aitken

Organizations

  • Yale University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Antigens
  • Arbovirus Infections
  • Arboviruses
  • Biological Factors
  • Blood
  • Blood Cells
  • Body Fluids
  • Cells
  • Culture Techniques
  • Dengue
  • Leukocytes
  • Pathogenesis
  • Rhesus Monkeys
  • Vaccines
  • Virus Diseases
  • Viruses
  • Yellow Fever

Fields of Study

  • Biology
  • Medicine

Readers

  • Infectious Disease/Epidemiology
  • Molecular Genetics
  • Vector-Borne Disease and Entomology

Technology Areas

  • Biotechnology