The Interaction of Pseudomonas Toxins with Epithelial Cell Membranes: A Primary Stage in the Pathogenesis Sequence of Cellular Intoxication
Abstract
During the 1980-81 contract period the following research information has been discovered: The degree of purity of a given Pseudomonas toxin A preparation is not necessarily related to the potency of the effect of that toxin on the isolated turtle bladder--even when the same toxin A preparation has proven to be a more potent pathogenetic agent in mammalian host cell systems. There are parallelisms between the inhibitory effects of toxin A and those of chlorpromazine (an adenylate cyclase inhibitor) on Na transport as well as anion transport. The preparation from turtle bladder cells of isolated, uniformly oriented apical membrane vesicles (right side out or inside out) with the ion transport functions still intact could provide a susceptible sub-cellular host system in which any toxin-induced changes in ion transport rates or in maintained transmembrane gradients of ion concentration could be direct and exclusive consequences of primary plasma membrane alterations.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 1981
- Accession Number
- ADA130573
Entities
People
- W. A. Brodsky
Organizations
- Icahn School of Medicine at Mount Sinai