Saint Louis Encephalitis Temperature-Sensitive Mutants.
Abstract
Saint Louis encephalitis temperature-sensitive mutants derived by induction with 5-azacytidine and 5-fluorouracil have been examined to determine complementation grouping, growth characteristics at several temperatures and virulence for mice. The results indicate that a maximum of five tentative complementation groups may be established. One mutant has been placed in groups I and II and may represent a double mutant. Examination of the ability of mutants to grow at 30 C, 40 C or 37 C indicates that some mutants are severely restricted at the nonpermissive temperature (40 C) while others are less so. Virulence studies show a separation between a-virulent mutants and those with a virulence quite similar to wild type. Mutants with a restricted growth in cell culture and a mutant with intermediate growth in cell culture were of greatly reduced virulence and behaved similarly when injected into mice.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1980
- Accession Number
- ADA131107
Entities
People
- Thomas A. Brawner