The Effects of Various Levels of Ascorbic Acid Intake Upon Oral Wound Healing in Guinea Pigs.

Abstract

The purpose of this study was to evaluate the effects of varying levels of orally administered ascorbic acid on wound healing in guinea pig oral mucosa. Forty five Murphy/Hartley guinea pigs were randomly placed into four groups and fed an ascorbic acid deficient diet for 2 weeks. Each group of animals then received a daily oral supplement of the following doses of ascorbic acid: 0.5 mg., 5.0 mg., 50 mg., and 250 mg. All animals were weighed twice a week. Twenty eight days later, a standardized wound was made in the premaxilla. On day 36, all animals were sacrificed. Blood samples were evaluated for levels of ascorbic acid. Thirty nine samples showed insignificant levels of vitamin C, 32 of which showed no ascorbic acid. Block sections of the premaxillary wound site were processed for histologic evaluation. Cross sections of the healing wounds were stained with H+E and Masson and were evaluated using a quadratic test grid. Results revealed that varying the levels of ascorbic acid had no significant effect on wound healing at 8 days (P>.05).

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1983
Accession Number
ADA132481

Entities

People

  • Andrew E. Stanya

Organizations

  • Air Force Institute of Technology

Tags

DTIC Thesaurus Topics

  • Adrenal Glands
  • Blood
  • Blood Vessels
  • Body Weight
  • Bone Diseases
  • Breakpoint Temperature
  • Cells
  • Chemistry
  • Connective Tissue
  • Diseases And Disorders
  • Fish
  • Hematologic Diseases
  • Microvessels
  • Nutrition Disorders
  • Surgery
  • Teeth
  • Vitamin C

Fields of Study

  • Agricultural and Food sciences
  • Biology
  • Medicine

Readers

  • Analytical Chemistry
  • Exercise and Sports Science.
  • Immunology