The Role of recA Protein in the Multiplicity Reactivation Pathway of Phage T4.
Abstract
The effect of two recA alleles on the multiplicity reactivation pathway of mitomycin C damaged bacteriophage T4 was studied. It was shown that both primary functions attributed to the enzyme, that of catalyzing homologous pairing and strand transfer reactions of joint molecule formation and that of a highly specific protease, are required for full function of the pathway. Additionally, the T4 gene uvsX, reported to code for a recA like protein was not found to affect the efficiency of MR. In the course of the investigation, it was noted that starving the host cells prior to phage infection appeared to induce MR. Evidence is presented that this induction occurs by a recA mediated mechanism.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1983
- Accession Number
- ADA132508
Entities
People
- Ronald Patrick Mccreary
Organizations
- Air Force Institute of Technology