Tricothecenes Mycotoxin Studies
Abstract
The purposes of the research are to elucidate the metabolism and toxicity of tricothecene mycotoxins, identify and synthesize their active metabolites and identify and synthesize compounds to block or reduce their toxicity. An efficient procedure for purification of anguidine has been developed to provide uniformly pure samples for all phases of the program. Tritiated anguidine has been prepared for use in metabolic studies, and metabolites have been synthesized for use as standards in identification of metabolites. Synthesis of nivalenol is proceeding. Conditions have been established for study in vitro of hepatic microsomal metabolism and for isolation, identification and quantitation of metabolites of anguidine. Metabolism occurs rapidly in the system; the three metabolites detectable by fluorescence on TLC are present within 15 minutes. Toxic effects of anguidine given in lethal or sublethal doses to mice and rats by gavage, parenteral or topical cutaneous application have been studied by histopathologic and hematologic examination. The LD50 has been measured, and sublethal doses that give consistent effects on the hematopoetic and lymphoid systems have been established.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1983
- Accession Number
- ADA132929
Entities
People
- A. E. Rogers
- G. H. Buchi
- M. A. Marletta
- P. M. Newberne
- W. R. Roush
Organizations
- Massachusetts Institute of Technology