Immunochemical Methods for Quantitation of Vitamin B6

Abstract

A procedure is described which proposes schemes for determining the total of all B6 vitamins in acid-hydrolyzed samples utilizing a radio- immunoassay (RIA) or an enzyme-immunoassay (EIA). Sample preparation is similar for both RIA and EIA. Two specific antibodies (antipyridoxine and antipyridoxamine) are employed to determine pyridoxamine, a portion of the sample is reduced with sodium borohydride. Pyridoxal is determined by difference between pyridoxine before and after reduction. The results indicate that two procedures have been developed which are selective for pyridoxamine (the fluorescent enzyme immunoassay and the spin immunoassay) and one assay which is equally sensitive to pyridoxine and pyridoxamine (the radio-immunoassay).

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Document Details

Document Type
Technical Report
Publication Date
Sep 30, 1981
Accession Number
ADA133108

Entities

People

  • David L. Brandon
  • Joseph W. Corse

Tags

Communities of Interest

  • C4I

DTIC Thesaurus Topics

  • Carrier Proteins
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Immune Serums
  • Liquid Chromatography
  • Measurement
  • Organic Chemistry
  • Proteomics

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  • Analytical Chemistry
  • Molecular and Cellular Biochemistry