Isoelectric Focusing Patterns of Staphylococcal Exfoliative Toxin

Abstract

Multiple components in two antigenic variants of staphylococcal exfoliative toxin were demonstrated by isoelectric focusing in polyacrylamide gel. The main components had an isoionic point of pH = 7.0 while other components ranged from pI = 4.5 to pI = 8.5. Each individual component was shown to possess full biologic and serologic activity after focusing. Isoelectric focusing in the presence of 8 M urea also showed microheterogeneity in exfoliative toxin preparations. The various components appear to be reversible conformers, possibly raised by exposure to the pH gradient of the ampholines.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1983
Accession Number
ADA133778

Entities

People

  • Anna D. Johnson-winegar
  • Leonard Spero

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Albumins
  • Alcohols
  • Amino Acids
  • Amphoterism
  • Animals
  • Biochemistry
  • Chemical Synthesis
  • Chemistry
  • Electrodes
  • Gel Electrophoresis
  • Infection
  • Infectious Diseases
  • Laboratory Animals
  • Macromolecules
  • Materials
  • Molecular Weight
  • Proteins

Fields of Study

  • Biology

Readers

  • Microbial Pathology
  • Polymer Science and Technology