Detection of T-2 Toxin by an Improved Radioimmunoassay
Abstract
T-2 toxin in serum, urine, and saline was analyzed by a modified radioimmunoassay procedure. The specimens were added directly to the assay tubes without extraction steps. The reaction between antibody and ligands was optimal at 1-h. Albumin-coated charcoal was used to separate bound from free radioactivity. Quenching, which occurred with hemolyzed specimens, was corrected by a wet oxidation process with 60% perchloric acid and 30% hydrogen peroxide. The shorter incubation times resulted in an assay that takes less than 6 h to complete. The average affinity constant of the antibody (K sub m) was 1.75 x 10 (expn 10) liters/mol. The sensitivity was 1 ng per assay or 10 ng/ml. Among the other trichothecenes tested, only H-T-2 cross-reacted significantly (10.3%).
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 1983
- Accession Number
- ADA133843
Entities
People
- David L. Bunner
- Fun Sun Chu
- Joanne Beheler
- Paulito A. Fontelo
Organizations
- United States Army Medical Research Institute of Infectious Diseases