Evaluation Studies of the DEN-2/S-1 Vaccine.

Abstract

The purpose of this study was to determine how much dengue 2 and dengue 4 viruses were required to infect rhesus monkeys. To this effect, groups of five dengue antibody free animals were inoculated subcutaneously with serial 10 fold dilutions of dengue 2 (PR159) and dengue 4 (H241) viruses. Virus titrations were carried out simultaneously by direct plaque assay in LLC-MK2 cells, by mosquito inoculation using both Aedes aegypti and Toxorynchites amboiensis and in the monkeys. Blood samples for serology were taken at 30 and 60 days post infection. Neutralization tests revealed that 9.5 mosquito infectious do se (50) (MID)50 of dengue 2 and 22 MID(50) of dengue 4 were required to infect 50% of the monkeys. The data suggest that in receipients challenges with wild type virus, a minimum of 100 MID(50's) should be used as the virus challenge dose.

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Document Details

Document Type
Technical Report
Publication Date
Nov 01, 1982
Accession Number
ADA135802

Entities

People

  • E. Kraiselburd

Organizations

  • University of Puerto Rico

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Animals
  • Antibodies
  • Biomedical Research
  • Cells
  • Dilution
  • Infection
  • Inoculation
  • Laboratory Animals
  • Monkeys
  • Puerto Rico
  • Rhesus Monkeys
  • Security
  • Technical Information Centers
  • Test And Evaluation
  • Universities
  • Wound Infections

Fields of Study

  • Biology
  • Medicine

Readers

  • Virology (or Medical Virology).

Technology Areas

  • Biotechnology