A Competitive Displacement Assay to Detect Saxitoxin and Tetrodotoxin

Abstract

An assay is described which detects saxitoxin (STX) and tetrodotoxin (TTX) by their competitive displacement of 3H-saxitoxin from its receptor in rat brain membranes. The assay has a sensitivity of 0.15 ng STX/m1 and 0.8 ng TTX/m1 for buffer samples. The assay was also applied to detection of these toxins in unextracted human plasma and found to have a sensitivity of 0.5 ng STX/m1 and 0. 6 ng TTX/m1. The competitive displacement assay appears to be the most sensitive procedure yet for detection of STX and TTX.

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Document Details

Document Type
Technical Report
Publication Date
Dec 09, 1983
Accession Number
ADA135965

Entities

People

  • Paulito A. Fontelo
  • Stephen R. Davio

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Animals
  • Biomedical Research
  • Cells
  • Cellular Structures
  • Clinical Laboratories
  • Detection
  • Displacement
  • Errors
  • Filters
  • Fish
  • Infectious Diseases
  • Laboratory Animals
  • Materials
  • Membranes
  • Poisoning
  • Public Health
  • Standards

Fields of Study

  • Biology

Readers

  • Cardiovascular Physiology
  • Molecular Genetics