The Effects of Organophosphorus Anticholinesterase Compounds on Brain Glucose and Energy Metabolism.

Abstract

The purpose of these experiments was to determine the effect of an organophosphorus cholinesterase inhibitor, paraoxon, on glucose utilization and the levels of intermediary metabolites in brain. The 24 hr. I.V. LD(50) of paraoxon in rats was 0.764 + or - 0.032 micromol/kg. A dose of 0.8 of the LD(50) did not result in any evidence of hypoxia in rats up to 2 hr. post-injection. The levels of glucose, pyruvate, glutamate, lactate, ATP, and phosphocreatine were determined in the following brain areas: cortex, midbrain, thalamus-basal ganglia, cerebellum and brain stem at 2, 10, 32 or 128 min. after administration of 0.8 or 0.5 of the LD(50) of paraoxon. The only consistent change observed was an elevation of tissue glucose. Injection of 0.8 of the LD(50) of paraoxon resulted in a significant depression in glucose utilization in the cortex for up to 30 min. However this decrease was not observed at 128 min. when the peripheral cholinergic effects were also absent. The diminished glucose utilization may be the cause of the increase in brain glucose observed. The decrease in glucose utilization may be due to an increase in inhibitory cholinergic neuronal activity.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1983
Accession Number
ADA137819

Entities

People

  • A. L. Miller
  • M. A. Medina

Organizations

  • University of Texas Health Science Center at San Antonio

Tags

DTIC Thesaurus Topics

  • Arteries
  • Biomedical Research
  • Blood
  • Blood Gases
  • Body Temperature
  • Brain
  • Brain Stem
  • Central Nervous System
  • Cerebellum
  • Enzyme Inhibitors
  • Laboratory Animals
  • Metabolism
  • Metabolites
  • Nervous System
  • Neurons
  • Organophosphorus Compounds
  • Thalamus

Fields of Study

  • Biology
  • Medicine

Readers

  • Cardiovascular Physiology
  • Neurotoxicology
  • Nuclear and Radiation Engineering.