Partial Purification and Characterization of Restriction Endonuclease from Neisseria meningitidis.

Abstract

A restriction endonuclease, NmeI, present in Neisseria meningitidis DRES W34 was studied. The enzyme was partially purified by passing through a blue 2 cross-linked agarose column; no contaminating nucleases remained detectable. This enzyme cleaved phage lambda, adenovirus type 2 (Ad 2) and phi x 174 DNA but did not cleave simian virus 40 (SV40) DNA. It had an absolute requirement for Mg(2+) for its activity and was inhibited by high concentrations of sodium chloride or magnesium chloride. NmeI activity was completely abolished after one hour of incubation at 65 C. S-adenosyl-L-methionine and ATP had no effect on its activity suggesting that NmeI is a type II restriction endonuclease enzyme.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Dec 01, 1983
Accession Number
ADA138308

Entities

People

  • A. R. Bhatti
  • R. Sparling

Organizations

  • Defence Research and Development Canada

Tags

DTIC Thesaurus Topics

  • Acids
  • Adenoviruses
  • Deoxyribonucleic Acids
  • Electrophoresis
  • Enzymes
  • Fish
  • Gel Electrophoresis
  • Incubation
  • Materials
  • Methionine
  • Microbiology
  • Microorganisms
  • Nucleic Acids
  • Phosphodiesterases
  • Recognition
  • Sugar Alcohols
  • Viruses

Fields of Study

  • Biology

Readers

  • Cardiovascular Physiology
  • Immunology
  • Molecular Genetics