Pathogenesis of Salmonellosis: Salmonella Exotoxins
Abstract
The first manuscript describes two new in vitro assays, that were developed to facilitate future studies of the characteristics of Salmonella toxin and its involvement in the pathogenesis of salmonellosis. One of the rapid, in vitro assays measures biological activity in terms of Salmonella toxin's capacity to stimulate adenylate cyclase in Chinese hamster ovary cells. The second manuscript describes our efforts to use the ELISA and CHO cell assay to study the effect of several cultural parameters on the synthesis and releases of this heat labile, choleragen-like toxin from growing Salmonella cells. The study describes the production and release of Salmonella toxin in a classic growth curve experiments with and without the addition of mitomycin C (MTC). We had reported earlier that MTC increased the concentration of CHO cell toxin in crude culture filtrates of Salmonella species (12, 16) but the mechanism was unclear. The remainder of the annual report summarizes significant and successful observations made during this period that have not yet been organized into publication format. The first section reflects a study of toxin production in a chemically defined medium and the stimulatory effects of vitamins, amino acids, and carbon sources on toxin synthesis. This study may aid in the development of a simple, inexpensive culture medium for toxin production that will not complicate purification work. We have successfully used such a medium for large scale cholera toxin for several years.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 08, 1982
- Accession Number
- ADA138758
Entities
People
- J. W. Peterson
Organizations
- University of Texas Medical Branch