Immunochemical Identification of Newcastle Disease Virus Structural Proteins,
Abstract
The immunochemical reactivity of the structural polypeptides of two strains of Newcastle Disease Virus (La Sota and B1), when reacted with hyperimmune polyclonal antisera, has been studied. Methods are described for the separation of NDV proteins by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (reduced and non-reduced), the electrophoretic transfer of separated proteins to nitrocellulose paper, and the subsequent identification of NDV antigenic determinants by a sensitive enzyme-linked immunosorbent assay. Using these methods, a number of new, minor polypeptides have been detected which hitherto have been described.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1984
- Accession Number
- ADA138830
Entities
People
- R. E. Fulton
- R. Shew
Organizations
- Defence Research and Development Canada