Pathogenesis of Salmonellosis: Salmonella Exotoxins

Abstract

We are now using several rapid, in vitro methods to quantitate Salmonella toxin contained in culture filtrates and sonicated cell preparations. Included among these newer techniques are the Chinese hamster ovary (CHO) cell alongation assay, the CHO floating cell assay, the pigeon erythrocyte lysate assay, two new agar plate techniques for detecting toxin from Salmonella colonies, and an enzyme linked immunosorbent assay. Detailed explanations of each assay are presented later in this section. The behavior of Salmonella toxin in each of these assays is indistinguishable from that of purified cholera toxin, and we have adopted the policy of expressing Salmonella toxin values in terms of chloera toxin equivalents (ng/ml) based on direct comparison in each assay with a set of cholera toxin standard solutions. Antigenic and biologic similarities of Salmonella toxin to chloera toxin have been reported previously.

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Document Details

Document Type
Technical Report
Publication Date
Mar 08, 1982
Accession Number
ADA139007

Entities

People

  • Johnny W. Peterson

Organizations

  • University of Texas Medical Branch

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Bacteria
  • Bacteriophages
  • Biological Toxins
  • Biomedical Research
  • Cell Line
  • Cells
  • Chemistry
  • Culture Media
  • Epithelial Cells
  • Escherichia Coli
  • Fungi
  • Gammaproteobacteria
  • Liquid Chromatography
  • Medical Personnel
  • Mucous Membrane
  • Rodents

Fields of Study

  • Biology

Readers

  • Microbial Pathology
  • Systems Analysis and Design
  • Toxicology/Environmental Toxicology