Evaluation of the Stability of Hemoglobin in Preparation and Storage by Electron Spin Resonance.
Abstract
Techniques have been developed to quantitatively measure the methemoglobin content of hemoglobin samples using electron spin resonance spectroscopy. It is also possible to follow the buildup of the hemichromes as the methemoglobin decays. This technique was applied to hemoglobin supplied by Fischer and also to samples prepared for us by LAIR. The Fischer samples came sealed in glass ampules. They contained relatively large amounts of hemichromes but their hemichrome content seemed independent of storate times. Their hemichrome signals also fell into two distinct types. The LAIR samples, supplied in blood-packs, had a well defined halflife for methemoglobin decay of 39.1 + or - 3.2 days. Their hemichromes' singals started out small but increased as the methemoglobin decayed. They did not also fall into the simple two type pattern of the Fischer samples. The differences in stability and hemichrome content between the Fischer and LAIR hemoglobin samples must be due to differences in preparation and storage conditions.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1981
- Accession Number
- ADA139220
Entities
People
- R. J. Myers
Organizations
- University of California, Berkeley