The Role of Newly Discovered Exotoxin (S Toxin) in Pseudomonas aeruginosa Infections
Abstract
The goal is to determine the role of exotoxin S in humans infected with Pseudomonas aeruginosa and develop vaccines to reduce the morbidity and mortality associated with these infections. During the period we have: (1) Developed a specific assay for S which allows us to distinguish between the enzymatic activities of toxins A and S. (2) Utilized the above assay to determine the percentage of clinical isolates of P. aeruginosa which produce S. (3) Purified analytical amounts of S, determined its molecular weight and used the pure S to raise specific S antibodies in rabbits. (4) Developed a defined medium for the production of S by P. aeruginosa strain 388-6. (5) Continued studies on the large scale purification of S. (6) Purified several large batches of P. aeruginosa toxin A and diphtheria toxin.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1980
- Accession Number
- ADA139493
Entities
People
- Barbara H. Iglewski
- Michael R. Thompson
Organizations
- Oregon Health & Science University