The Response of Rats to Cutaneous Dosing with Trichothecene Mycotoxins,

Abstract

T-2 toxin and diacetoxyscirpenol (DAS) formed deep necrotic ulcers on rat skin at doses of 2 micrograms and 6 micrograms respectively. Verrucarin A and Roridin A were relatively ineffective at producing skin lesions. The percutaneous LD50 of T-2 or DAS was found to be well in excess of 10 mg/kg. DMSO had no marked effect in increasing wound severity or systematic toxicity as opposed to ethyl acetate as the application vehicle. No synergism between T-2 and DAS was found. Histological examination of skin lesions revealed the typical inflammatory reaction that is also caused by thermal burns or other necrotising agents. Thin layer chromatography of the toxins is described, as a method to check purity and stability.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1983
Accession Number
ADA139548

Entities

People

  • H. D. Crone

Organizations

  • Defence Science and Technology Group

Tags

DTIC Thesaurus Topics

  • Body Weight
  • Burns
  • Chemical Synthesis
  • Chemistry
  • Chromatography
  • Epidermis
  • Fungi
  • Liquid Chromatography
  • Macrocyclic Compounds
  • Materials
  • Organic Chemistry
  • Organic Materials
  • Rodents
  • Security
  • Thermal Burns
  • Tissues
  • Toxicity

Fields of Study

  • Biology

Readers

  • Cardiovascular Physiology
  • Mycotoxin ecology in Amazonian ecosystems.
  • Trauma Surgery or Emergency Medicine.