Histocompatibility Typing.
Abstract
In order for bone marrow transplantation to be an effective and successful therapeutic measure in the treatment of irradiation casualties, the donor marrow cells and the recipient must be matched for HLA antigens. Matching for antigens encoded by HLA-D appears to be most critical, however, we are just beginning to comprehend the complexity and importance of the HLA-D region. This contract was established to systematically analyze those HLA-D antigens most important for bone marrow transplantation and to develop and implement methods for matching large numbers of unrelated individuals for clinical bone marrow transplantation. We describe herein our progress in using cellular methods for elucidating the genetics of the HLA-D region and the initiation of biochemical approaches to clinical transplant matching. These methods include primed lymphocyte typing (PLT) generation of human T-lymphocyte clones (TLC's) that recognize histocompatibility antigens, homozygous typing cells (HTC) characterization and peptide map characterization. During the upcoming contract period, we will continue our efforts in cellular immunology, and will also continue to develop parallel programs in the immunochemistry of human alloantigens and serologic recognition of HLA antigens by human monoclonal antibodies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 15, 1984
- Accession Number
- ADA139748
Entities
People
- C. K. Hurley
- D. D. Eckels
Organizations
- Georgetown University