Rapid Diagnosis of Arbovirus and Arenavirus Infections by Immunofluorescence.
Abstract
Polyvalent slides bearing a mixture of five cell cultures each infected with a different group B arbovirus, for use in the indirect immunofluorescence test, have been prepared. The viruses in the slide were: Banzi, Japanese encephalitis, Langat, Rocio and yellow fever. The slides gave positive reactions when tested with hyperimmune mouse sera including 6 of 7 sera for viruses not represented in the mixture. It seems advisable, however, to prepare two different complementary sets of group B polyvalent slides if a complete or nearly complete coverage is to be achieved. Inactivation of yellow fever virus-carrying spot-slides can be accomplished by immersion in dilute betapropiolactone however, the quality of the slides may be impaired by this procedure. The immunofluorescence test with spot-slides prepared with viral isolates submitted for identification has continued to prove a time and labor saving procedure for antigenic grouping of strains; the method has been used with strains origination in Fiji, Uganda and South Africa.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1981
- Accession Number
- ADA140101
Entities
People
- J. Casals-ariet
Organizations
- Yale University