Genetic and Physical Structures of Salmonella-coli Phage Hybrids and Development of New Generalized Transducing Hybrid Phages for E. coli.
Abstract
The frequency of hybrid formation between Salmonella phage P22 and coli mutator phage Mu is extremely low. Therefore we had to exert enormous effort to isolate hybrids. The hybrid MuimmP22 carries the entire late genes of Mu phage and the early genes, at least the c to att region of P22 phage. MuimmP22 lysogens are sensitive to P22 infection whereas P22 lysogens are immune to MuimmP22 infection because P22 has in addition to the c region the 2nd immunity (Im) region carrying the anti-repressor (ant) gene which can inactivate the c repressor. We also isolated MuimmP22dis hybrid which carries both the c and Im regions of P22 and is dismune over MuimmP22 lysogen. Although the entire late genes of MuimmP22 hybrid class are derived from Mu phage, MuimmP22 hybrid class infects a smooth P22-sensitive host but not a rough Mu-sensitive host WR4027.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1983
- Accession Number
- ADA140144
Entities
People
- N. Yamamoto
Organizations
- Hahnemann University Hospital