In vitro Studies of Sandfly Fever Viruses and Their Potential Significance for Vaccine Development.
Abstract
The structural and nonstructural polypeptides synthesized by several serologically distinct strains of sandfly fever viruses have been studied by immuno-precipitation and SDS-polyacrylamide gel electrophoresis. The resulting profiles show that a similarity exists in the number and molecular weights of the major structural and nonstructural proteins, but that most strains are distinguishable by this procedure. A similar analysis of six strains of Punta Toro virus which differ markedly in their virulence for laboratory animals has demonstrated that differences are not detectable in internal or nonstructural polypeptides, but that minor differences are seen in the surface glycoproteins. The antigen specificities of a panel of monoclonal antibodies produced against Punta Toro virus have been determined by immunoprecipitation and acrylamide gel analysis. RNA extracted from Karimabad virus-infected cells and added to rabbit reticulocyte lysates directs the synthesis of the major nonstructural protein (31K) and the nucleocapsid protein (24K), as well as several other proteins which have not been detected in infected cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 1981
- Accession Number
- ADA140162
Entities
People
- J. F. Smith
Organizations
- University of Maryland, Baltimore