The Collection, Purification, and Cryopreservation of Canine Peripheral Blood Mononuclear Cells Obtained as a By-Product of Platelet Apheresis Procedures.
Abstract
An average of 1.69 X 10 to the 9th power white blood cells was collected from each of 11 Beagel dogs during 169 routine platelet apheresis procedures using a Haemonetics Model 30 Blood Cell Separator. Seventy-four percent of the collected white blood cells were mononuclear cells. Treatment of the mononuclear cells with ficoll-hypaque to remove the granulocytes and red blood cells resulted in a loss of 25% of the mononuclear cells. After freezing with 10% DMSO, whether as buffy coats or after ficoll-hypaque treatment, thawing and washing, about 70% of the mononuclear cells were recovered as viable cells. Recovery values of viable mononuclear cells were similar whether there was rapid addition of the cryoprotectant and freezing at 2-3 C/minute in a -80 C mechanical freezer or slow addition of the cryoprotectant and freezing at 1 C/minute in a controlled-rate freezer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 23, 1983
- Accession Number
- ADA140404
Entities
People
- A. D. Gray
- A. J. Melaragno
- A. J. Roy
- J. Dittmer
- L. T. Lavigne
Organizations
- Boston University