A Microaffinity Assay for Plasma Fibronectin.
Abstract
The quantitation of fibronectin in human plasma has been based exclusively on immunochemical methods to-date. An alternate method is described which utilizes gelatin-coated Sepharose 4B beads to bind the fibronectin present in plasma, and by a series of washings aided by centrifugation to remove the unbound material. Elution of the protein with 6 M urea followed by a colorimetric reaction with the Bio-Rad protein dye reagent (Coomassie Blue G-250) produces a quantitative estimation of fibronectin in plasma. Twenty-two human plasma samples from healthy volunteers were analyzed by the microaffinity assay and by a commercially available immunoturbidimetric assay kit. There was a poor correlation (r = 0.5032) between the two assays. The concentration of fibronectin in plasma was 356 + or - 87 micrograms/ml by the microaffinity assay, and 270 + or - 57 micrograms/ml by the immunoturbidimetric method.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 25, 1983
- Accession Number
- ADA140420
Entities
People
- A. L. Griffith
- B. Perilli-palmer
- C. R. Valeri
- N. Catsimpoolas
Organizations
- Boston University