Factors Governing the Subcellular Distribution of Indium-111 in Human Platelets.
Abstract
The subcellular distribution of indium-111 (In-111), and the effect of the metabolic inhibitors rotenone and 2-deoxyglucose on its uptake, retention, and subcellular distribution, have been investigated in human platelets using techniques which permit the maintenance of dense body integrity during fractionation. As with chromium-51 (Cr-51), the In-111 label appears to be located principally in the cytosolic (soluble) fraction. Equilibrium dialysis studies suggest that only 10-20% of the In-111 is associated noncovalently with non-microsomal proteins. There appears to be a relationship between the metabolic pool of nucleotides and the uptake and retention of In-111, since incubation of platelets at 37 C with metabolic inhibitors prior to labeling with In-111 reduces the amount of label taken up when compared to platelets incubated at 22 C.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 21, 1982
- Accession Number
- ADA140423
Entities
People
- C. R. Valeri
- C. Rushin
- J. J. Vecchione
- J. L. Costa
Organizations
- Boston University