Measurement of the Distribution of Indium-111 on Human Plasma Proteins Using Chromatography and Immunoprecipitation.
Abstract
The distribution of radioactivity on plasma proteins labeled by addition of Indium-111-oxine to citrated plasma was investigated. Analysis of plasma proteins separated on Sephadex G-200 columns showed that 23-36% of the In-111 was associated with proteins with molecular weight greater than 20,000 daltons and the remaining In-111 was associated with proteins with molecular weight less than 100,000 daltons, presumably transferrin. Affinity chromatography experiments showed that less than 2% of the radioactivity was associated with albumin. Further identification of the labeled proteins and quantitation of associated radioactivity was performed by precipitating specific proteins with antibodies. These studies showed that the In-111 was distributed on transferrin (54-76%), fibrinogen (11-24%), IgM (8-20%), C3 (10-21%), and haptoglobin (3-8%). In-111 associated with fibrinogen, IgM, and haptoglobin was overestimated in some experiments due to binding of In-111 labeled C32 to the antigen-antibody precipitates.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 13, 1982
- Accession Number
- ADA140458
Entities
People
- A. J. Melaragno
- B. J. Weiblen
- C. R. Valeri
- N. Catsimpoolas
Organizations
- Boston University