Mechanisms of Protective Immunogenicity of Microbial Vaccines of Military Medical Significance.

Abstract

Clinical studies of Q fever vaccine have been undertaken with screening of volunteers for humoral and cellular immune reactivity to specific Q fever antigens. A rapid and simplified immunofluorescent procedure has been developed for serodiagnosis of Q fever and tularemia suitable for field use. Excellent correlation exists between the results of this procedure and standard complement fixation testing for diagnosis of Q fever. Lot-to-lot variability of phase I Q fever vaccine has been assessed by in vitro lymphocyte transformation testing of immune guinea pigs. There is a consistent response between lots of vaccine by this technique and no evidence of endotoxin contamination in the vaccine. Delayed hypersensitivity skin testing of guinea pigs with Q fever antigen has revealed granulomatous changes with giant cell prominence. The immunologic basis of the granulomatous response is under analysis.

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Document Details

Document Type
Technical Report
Publication Date
Jan 30, 1981
Accession Number
ADA140715

Entities

People

  • M. S. Ascher

Organizations

  • University of California, Irvine

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Blood
  • Bone Marrow
  • Cells
  • Cellular Structures
  • Endoplasmic Reticulum
  • Immune Serums
  • Laboratory Animals
  • Macrophages
  • Medical Personnel
  • Q Fever
  • Rodents

Fields of Study

  • Biology
  • Medicine

Readers

  • Immunology

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech