Stability and Function of Granulocytes Isolated by Counterflow Centrifugation and Discontinuous Density Gradients of Ficoll-Hypaque.
Abstract
Granulocytes isolated by counterflow centrifugation elutriation (CCE) were compared with those isolated by dextran sedimentation, centrifugation with Ficoll-Hypaque, and lysis of residual red cells (SFWL). They were also compared with granulocytes isolated on discontinuous density gradients using two densities Ficoll-Hypaque (DFDG). The cells were stored at 4 C for 72 hours and aliquots measured for phagocytic indices (particle ingestion), membrane integrity (fluorescein-diacetate-ethidium bromide) volume (electronic sizing) and membrane function (superoxide anion). CCE and SFWL prepared granulocytes were more stable than DFDG cells, The SFWL method is preferred over CCE because of its simplicity and the high quality and purity of the isolated granulocytes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 15, 1984
- Accession Number
- ADA142516
Entities
People
- F. J. Lionetti