Karnovsky-Osmium as a Primary Fixative for Electron Microscopy.

Abstract

A combined Karnovsky-osmium tetroxide primary fixative is proposed for transmission electron microscopy. This fixative, hypothetically, seems to overcome the drawbacks of sequential fixation techniques. Evaluation of this combined fixative reveals very acceptable fixation of hepatocytes, lymphocytes, and fibroblasts, as well as various organelles within these cells. (Author)

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1983
Accession Number
ADA142671

Entities

People

  • G. M. Buchanan

Organizations

  • Armed Forces Radiobiology Research Institute

Tags

DTIC Thesaurus Topics

  • Cells
  • Chemical Synthesis
  • Chemistry
  • Electron Microscopy
  • Electrons
  • Elements
  • Endoplasmic Reticulum
  • Epithelial Cells
  • Fibroblasts
  • Lymphocytes
  • Microscopy
  • Organelles
  • Osmium Compounds
  • Radiobiology
  • Rodents
  • Tetroxides
  • Transmission Electron Microscopy

Readers

  • Molecular Biology and Genetics
  • Oncology and Biomarker-Based Cancer Detection.
  • Systems Analysis and Design

Technology Areas

  • Microelectronics