The Effects of EDTA (Ethylenediaminetetraacetic Acid) and Sonication on the Disaggregation of Oral Bacteria,
Abstract
The dispersive effects of ethylenediaminetetraacetic acid (EDTA) and ultrasound on bacterial aggregates in an anaerobic environment were studied. Polled supragingival plaque samples were placed into reduced transport fluid (RTF) containing 1 mmol, 10 mmol, 100 mmol EDTA, or EDTA-free RTF for dispersion, and colony forming units (cfu) were determined after culture at 37 C for 72 hours. Mean colony forming units were increased 42.7% and 54.4% respectively in 1 mmol and 10 mmol concentrations of EDTA and decreased when plaque was dispersed in 100 mmol concentrations of EDTA. Fusobacterium nucleatum was cultured in broth medium, placed into RTF containing no chelating agent, and sonicated for either 0, 5, 15, or 30 seconds. Sonication at the lowest power setting produced a statistically significant (p<.01) increase in CFU after 5 seconds. This trend did not continue when time of sonication was increased. The effect of sonication at medium or high power settings was variable, and factors affecting variability are discussed. EDTA and sonication may enhance the quantitation of oral microorganisms.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1984
- Accession Number
- ADA143396
Entities
People
- C. E. Hawley
- J. W. Vincent
- M. B. Dayoub
Organizations
- United States Army Institute of Surgical Research