Development of Vaccines to the Mycotoxin T2
Abstract
An EIA assay system to detect and titer antibody to Mycotoxin T2 was standardized. A new rapid of the new method over the existing one accounts for its simple, less time-consuming procedure, which can be carried out at room temperature, with as high as a yield as 66%. T2-HS so developed was conjugated to derivatised sepharose-4B beads to make a column to affinity purify the antibodies to Mycotoxin T2. Affinity purified antibody to T2 was used for immunizing the mice for the development of anti-idiotypic antibodies. Three different immunization schedules were employed, such as, anti-T2 antibody conjugated to KLH, anti-T2 antibody conjugated to goat IgD, and anti-T2 antibody conjugated to monoclonal B6 anti-BALB/C IgD. Anti-idiotypic antibody was detected by EIA (competitive inhibition reaction by T2). Mice producing a titer of 1:800 to 1:20,000 were detected. Induction of leucopenia by T2 toxin in mice was examined. Optimum sublethal concentrations of T2 toxin and route of administration were standardized. Time course of white blood cell count after administration of toxin, was established. Effect of toxin on the other lymphatic organs was also examined.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 31, 1984
- Accession Number
- ADA145271
Entities
People
- Vijaya Manohar