Test of Suppressor Cell Activation in African Trypanosomes.
Abstract
Suppressor cells from trypanosome infected mice are activated by exposure to trypanosome factor(s) in vitro. Primed, helper T-cells are not required for activation. The pfc response following removal of 'helpers' or 'suppressors' measures the activity of splenic suppressors from infected mice. Suppressor cell activation occurs in the absence of direct suppression of helper cells, or effector cell activation. Stimulation of prostaglandin (PGE) synthesis evokes suppressor cell activity, and is assayed by the effect of PGE1, PGE2, and PG inhibitors on the mixed spleen pfc response. Glass adherent T-cells (GAT) added to non-adherent lymphocytes (NAL) at 24 or 48 hr. after initiation of culture suppressed DNA synthesis in NAL. The amount of PG (and metabolites) generated by NAL from initiation of culture to 48 hr. (by inhibition assay) appears to activate GAT in a manner analogous to that found when exogenous PGE2 is added. Synthesis of PG in spleen cell cultures and in macrophage enriched populations suggests that the products of GAT and macrophage suppress DNA synthesis in NAL in vivo.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1983
- Accession Number
- ADA145387
Entities
People
- A. C. Zahalsky
Organizations
- Southern Illinois University Edwardsville