Testing of Experimental Compounds against American Mucocutaneous and Cutaneous Leishmaniasis.

Abstract

Leishmania m. mexicana and L. b. panamensis served as standard subspecies for drug screening. Both amastigotes and promastigotes yielded suitable lesions for drug screening, and promastigotes grown in insect media were as infective as amastigotes. In Schneider's drosophila medium, subcultured promastigotes remained infective. Stationary phase cells were more infective in all media tested. Time to lesion development was staged and dose dependent. Using Pentostam and Glucantime to treat developed lesions of L. m. mexicana and L. b. panamensis, we showed that neither drug can cure these infections, leishmania b. panemensis was more sensitive to Glucantine, the SD50 and 90 for these subspecies was greater than that for visceral L. donovani infections. Using L. donovani in BALB/c mice, we showed that: fourteen day assays are highly reliable whether mice are inoculated IC or IV with 10 million splenic amastigotes or promastigotes.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 1981
Accession Number
ADA145486

Entities

People

  • J. S. Keithly

Organizations

  • Weill Cornell Medicine

Tags

DTIC Thesaurus Topics

  • Animals
  • Biomedical Research
  • Cell Line
  • Cell Nucleus
  • Cells
  • Contracts
  • Drinking Water
  • Infection
  • Inhibitors
  • Laboratory Animals
  • Leishmania
  • Leishmaniasis
  • New York
  • Stationary
  • Technical Information Centers
  • Universities
  • Wound Infections

Fields of Study

  • Biology

Readers

  • Parasitology and Pharmacology of Malaria.
  • Toxicology/Environmental Toxicology