Infectious Multiple Drug Resistance in the Enterobacteriaceae
Abstract
The applicability and limitations of examining clinical specimins with DNA hybridization technique for gnes encoding enterotoxins was examined using 24 heat-labile and heat-stable (LT+ST+), 17 heat-labile (LT+ST), and 22 heat-stable (LT-ST+) enterotoxigenic Escherichia coli isolated in Thailand. Enterotoxigenic E. coli were identified with the Y-1 adrenal and suckling mouse assays. All were homologous and thus identifiable with radiolabled fragments of DNA encoding for heat-labile toxin (LT) or heat-stable toxins of porcine (ST-P) and human origin (ST-H). LT-ST+ strains from rural Thailand were homologous with only ST-H and not ST-P, while strains isolated in Bangkok were homologous with either ST-H, ST-P, or both. The hybridization technique detected DNA homologous with the three probes in bacterial growth of all stools from patients with diarrhea from whom enterotoxigenic E. coli were isolated and in enterotoxigenic E. coli-inoculated water containing other species of bacteria. The DNA hybridization assay is a useful technique for characterizing enterotoxigenic E. coli and identifying environmental sources of these enteric pathogens.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 1982
- Accession Number
- ADA146299
Entities
People
- Stanley Falkow
Organizations
- University of Washington