Morphological Changes in CHO and VERO Cells Treated with T-2 Mycotoxin. Correlation with Inhibition of Protein Synthesis
Abstract
Exposure of Chinese hamster ovary (CHO) and African green monkey kidney (VERO) cells to T-2 mycotoxin resulted in several morphological changes which appeared to be directly related to inhibition of protein synthesis, the basic in vitro mechanism of action of the toxin. These changes, which occurred in both cell types, included disassociation of ploysomes and mitochondrial cristae alterations. In addition, CHO cells displayed membrane bleb formations which were either a result of protein synthesis inhibition or a specific early pathological response. Bleb formations were not observed in VERO cells. Similar morphological changes, were found in both cell types exposed to established inhibitors of protein synthesis; puromycin or anisomycin.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 21, 1984
- Accession Number
- ADA146618
Entities
People
- Lynn R. Trusal
Organizations
- United States Army Medical Research Institute of Infectious Diseases