Liposome-Encapsulated Hemoglobin for Emergency Resuscitation.
Abstract
An oxygen-carrying resuscitation fluid has been developed based upon liposome-encapsulated hemoglobin (LEH). The hemoglobin so encapsulated carries oxygen with unimpaired efficacy. The LEH is stable in the presence of serum and red cells in vitro and displays rheological properties similar to red cells. When tested ex vivo, encapsulation provides some protection from the vasoconstrictive effects seen with stroma-free hemoglobin. In in vivo measurements the circulation persistence of LEH in mice exceeds a half lifetime of 6 hours, with animal survival extending beyond two months. An improved lipid formulation substitutes hydrogenated soy lecithin for dimyristoyl lecithin, providing a significant cost reduction and replaces dicetylphosphate with a negatively charged lecithin, to eliminate possible toxic effects. Preliminary data suggest that a major production scale-up can be achieved using commercially available equipment.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1984
- Accession Number
- ADA146794
Entities
People
- B. P. Gaber
- M. C. Farmer
Organizations
- United States Naval Research Laboratory