Structure of Antibodies by X-Ray Diffraction of Monomolecular Layers and by Nuclear Magnetic Resonance.
Abstract
It appears to be technically feasible, in principle, to obtain structural information on antibodies bound to lipid haptens in monomolecular films, using synchrotron radiation. The major obstacle to this approach is the limited availability of beam time on synchrotron sources. It has been found that significant structural information on the combining site of a monoclongal anti-spin lable antibody can be obtained using nuclear magnetic resonance combined with specific deuteration of selected amino acids. This opens the way for a rapid study of the relationship between structure and function of such antibodies when these studies are combined with modern recombinant DNA techniques. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1984
- Accession Number
- ADA146803
Entities
People
- H. M. Mcconnell
Organizations
- Stanford University