The Interaction of Coagulation Factor V and Vascular Endothelial Cells.
Abstract
Bovine aortic endothelium was studied in vitro with respect to synthesis of coagulation factor V. The bioassay revealed an increase of functional factor V, with time, in the culture supernatant, while solubilized cells were negative for functional factor V. Radioimmunoassay results indicated an increase, with time, of factor V/Va antigen in culture supernatants, and a constant level of antigen per cell from solubilized cells. Not all cells provided a positive fluorescence signal, endothelial cells stained differentially for factor V/Va with both the monoclonal and polyconal antibodies. Autoradiograms of electrophoresis gels with samples from immunoadsorbed S-culture products, and I-factor V/Va standards revealed labelled proteins with electrophoretic mobilities consistent with I-factor V/Va standards. Data from bioassay, radioimmunoassay and S-methionine incorporation each indicated factor V had been synthesized by cultured bovine aortic endothelium, while immunofluorescence data indicated factor V was associated with dendothelial cells. Additional, preliminary studies, associating factor V and the prothrombinase complex with endothelium, were accomplished through I-factor V/Va binding and S0-2238 chromogenic assays.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1984
- Accession Number
- ADA147411
Entities
People
- T. J. Cerveny
Organizations
- Air Force Institute of Technology