Genetic and Physical Structure of Salmonella-coli Phage Hybrids and Development of New Generalized Transducing Hybrid Phages for E. Coli.
Abstract
We have isolated unusual hybrid bacteriophages by recombining the evolutionary diverse bacteriophages Salmonella phage P22 and coliphages Mu80 or a mutator phage Mu. Genetic analysis of Mu80immP22 hybrid genomes led us to isolate high specialized transducing phages for argF and proAB genes of the E. coli chromosome although the parental phage Mu80, is a specialized transducing phage for the E. coli trp operon. Phage P22 can recombine with coli-mutator phage Mu. Genetic studies correlated with serological and host range analyses of MuimmP22 hybrids revealed that crossovers occurred at the tail fiber genes within the invertible G segment of Mu phage and the tail spike gene of P22 phage to form MuimmP22 hybrids. MuimmP22 hybrids infect hosts carrying the smooth host O-antigen (Man-Rha-Gal)n repeating unit which is specific receptor for adsorption of P22 phage. However, anti-P22 serum is unable to neutralize the MuimmP22 hybrids. This is probably because the contribution of P22 spike peptide to the hybrid tail fiber is to short to form P22 spike like structure and antigenicity. Anti-serum prepared for the G(+) orientation of Mu phage neutralized the MuimmPss hybrid at about a 1/10 neutralization rate of MuG(+) phage. Thus we suggested that MuimmP22 hybrid may carry the inverted (-) orientation of the G segment of Mu phage.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1984
- Accession Number
- ADA148613
Entities
People
- N. Yamamoto
Organizations
- Hahnemann University Hospital