The Effects of Specific Environmental Pollutants on the Biosynthetic Functions of Mammalian Cells In Vitro: A Search for Structure/Activity Relationships.
Abstract
A rabbit reticulocyte cell-free translation system was used to evaluate the effects of specific environmental pollutants on mammalian protein (globin) synthesis. Dose-response relationships were determined over a range from three to eight logs of concentration (generally 10 to the minus 12th to 10 to the minus 6th power M) for the following benzene and toluene derivatives: 1) benzene and various chloro and nitro - substituted benzenes; 2) assorted chloro, mononitro-, and monoamine-substitued toluenes; 3) all the dinitrotoluenes; and 4) 2,4,6 trinitrotoluene. The results indicate that no significant inhibition of protein synthesis occurs at concentrations below 0.00001 M for the majority of the agents tested. Solubility problems hindered testing at higher concentrations. Four of the compounds tested showed inverse relationships with greater inhibition at lower concentrations. Only one agent, 2,3 dinitrotoluene, displayed typical concentration-dependent inhibition but that only at concentrations greater than 0.00001 M. Inhibitory effects were observed for: 4-amino-3,5 dinitrotoluene, and 2,3 dinitrotoluene. The translation bioassay method is relatively simple and quick and shows good promise as a general procedure for evaluating chemical agents for toxicity.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 1980
- Accession Number
- ADA149184
Entities
People
- M. J. Fournier
- P. P. Parsons
Organizations
- University of Massachusetts Amherst