Spectrodensitometric Quantitative Determination of Trichothecenes in Water: Application to T-2 Toxin and T-2 Tetraol

Abstract

Trichothecene toxins were isolated from dilute aqueous solutions (1-4 mg/L) by adsorption on solid phase extraction (SPE) columns followed by elution with methanol. After thin layer chromatographic (TLC) separation, quantitative determinations were made by a spectrodensitometric method specific for trichothecenes. The extraction/quantitation procedure worked equally well for the relatively non-polar toxin, T-2, and the most polar trichothecene, T-2 tetraol. Recoveries were 95-100 percent for T-2 and 80-95 percent for tetraol. Originator-supplied keywords include: Densitometry, Field water and sanitation, Quantitative analysis, T-2 Tetraol, T-2 Toxin, and Trichothecenes.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1984
Accession Number
ADA149404

Entities

People

  • Charlotte M. Trout
  • Elizabeth P. Burrows
  • Evelyn H. Mcnamee

Organizations

  • United States Army Medical Research and Development Command

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Alcohols
  • Aqueous Solutions
  • Biological Warfare
  • Biological Warfare Agents
  • Biomedical Research
  • Chlorides
  • Extraction
  • Fungi
  • Liquids
  • Methanols
  • Phase
  • Recovery
  • Silica Gels
  • Solid Phases
  • Standards
  • Technical Information Centers
  • Water

Fields of Study

  • Chemistry

Readers

  • Analytical Chemistry
  • Mycotoxin ecology in Amazonian ecosystems.