Ultrasensitive Detection of Chemical Substances
Abstract
As stated in our previous report, we have concentrated our effort on reducing noise factor. In the previous quarter, experiments indicated nonspecific binding was due to polystyrene interactions between the bead and the plate. Recently we studied the interaction between beads whose surface carries one of three types of functional groups (amino, carboxyl, succinamide) and polystyrene plates treated so as to present surfaces with particular functional groups. These results are presented in Table 1. It can be seen that the greatest difference occurs when beads are presented to the polystyrene surface in a non- ionic solution giving noise levels of only 35 beads/sq cm. Variations are observed in other combinations of beads and plates, but nothing as dramatic as the incubations in deionized water. We therefore believe that most of the noise we have previously experienced has an ionic component. We are continuing to follow-up on these experiments to determine the ionic character of non-specific binding. We have also in this quarter developed a simple device for testing various plastics.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 30, 1984
- Accession Number
- ADA150049
Entities
People
- R. G. Dean
Organizations
- University of New Mexico