Nonspecific Resistance Induced by an Immunopharmacologic Agent Derived from Bordetella pertussis.
Abstract
Treatment of mice with Bordetella pertussis vaccine resulted in resistance to mouse adenovirus infection. Antiviral activity was associated with surface components of B. pertussis. Acellular fractions with antiviral activity were obtained by relatively gentle extraction methods, i.e., 1.0 M NaCl extraction or Waring blender treatment. B. pertussis lipopolysaccharide extracted by the Westphal procedure possessed antiviral activity. Lipoplysaccharide complexed with protein appeared to have more activity than purified lipopolysaccharide. B. pertussis vaccine did not induce cross-reacting antibodies with either neutralizing or protective activity toward mouse adenovirus. Peritoneal exudate cells obtained from mice treated with B. pertussis vaccine inhibited vesicular stomatitis virus multiplication in L-929 cells. Removal of adherent cells from the peritoneal exudate cell population increased the antiviral activity toward vesicular stomatitis virus. Mouse adenovirus migrates from the peritoneum to the blood within minutes after intraperitoneal inoculation. Infection with sublethal doses of mouse adenovirus resulted in a transient splenomegaly. Mice developed maximal neutralizing antibody titers after infection with as little as 10 plaque-forming units. Infection with lethal does of mouse adenovirus resulted in a fever response (hypothermia) before death.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 31, 1985
- Accession Number
- ADA150715
Entities
People
- A. L. Winters
- G. S. Sloan
- P. A. Leblanc
Organizations
- University of Alabama