Enzymatic Conversion of Red Cells for Transfusion.
Abstract
Our interest is in enzymatically converting type A and B erythrocytes to type O under conditions which render them useful in transfusion therapy. We are currently attempting such conversions of A cells using an alpha-N-acetylgalactosamindase (A-zyme) for chicken liver. This enzyme has been purified free of any detectable contaminating proteases, sialidase and other exoglycosidases with the the exception of alpha-galactosidase activity (8-10% of A-zyme) which is believed to be intrinsic and not due to a contaminating enzyme. It can remove A antigenicity from the red cell surface and is considerably more effective in doing so than comparable preparations of A-zyme from the earth worm and sheep. Results are encouraging in that they suggest that A antigenic sites resistant to the action of chicken liver A-zyme are not sterically inaccessible but given the proper enzyme and buffer conditions they too can be removed. Originator-supplied keywords include: Enzymatic conversion of type A cells to 0, Alpha-N-acetylgalactosaminidase or A-zyme, A-zyme treatment of type A cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 1985
- Accession Number
- ADA151720
Entities
People
- Joel Goldstein
Organizations
- New York Blood Center