A Study of the 5S Ribosomal RNAs of the Vibrionaceae
Abstract
The sequence of the 120 nucleotide bases of 5S ribosomal RNA (5SrRNA) has been determined for each of 23 eubacterial strains classified as species of the family Vibrionaceae. The methods employed included the resolution of limited enzymatic digests of end-labeled RNAs by high voltage polyacrylamide gel electrophoresis, as well as thin-layer chromatography. Several of the 5S rRNAs, for which the primary structures were determined, were also subjected to secondary structure analysis, employing nuclease S1, which hydrolyzes regions of nucleic acids not participating in the formation of helices, followed by resolution of the partial digests on thin sequencing gels. Sequence data obtained from this study were compiled and analyzed, using statistical methods and group-specific signature analyses, for the purpose of constructing a phylogenetic taxonomy of the Vibrionaceae. Dot matrix maps were generated for 5S rRNA sequences determined in this study, in order to analyze the occurrence, extent, and complexity of palindromic and repeated base sequences.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1984
- Accession Number
- ADA151986
Entities
People
- M. T. Macdonell
Organizations
- University of Maryland